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Abscisic acid promotes jasmonic acid biosynthesis via a ‘SAPK10-bZIP72-AOC’ pathway to synergistically inhibit seed germination in rice (Oryza sativa)
Yifeng Wang+, Yuxuan Hou+, Jiehua Qiu, Huimei Wang, Shuang Wang, Liqun Tang, Xiaohong Tong and Jian Zhang*
New Phytologist

Abstract

Abscisic acid (ABA) and Jasmonic acid (JA) both inhibit seed germination, but their interactions during this process remain elusive. Here, we report the identification of a “SAPK10-bZIP72-AOC” pathway, through which ABA promotes JA biosynthesis to synergistically inhibit rice seed germination.

Using biochemical interaction and phosphorylation assays, we show that SAPK10 exhibits auto-phosphorylation activity on the 177th serine, which enables it to phosphorylate bZIP72 majorly on 71st serine. The SAPK10-dependent phosphorylation enhances bZIP72 protein stability as well as the DNA binding ability to the G-box cis-element of AOC promoter,thereby elevating the AOC transcription and the endogenous level of JA.

Blocking of JA biosynthesis significantly alleviated the ABA sensitivity on seed germination,suggesting that ABA-imposed inhibition partially relied on the elevated level of JA.

Our findings shed a novel insight into the molecular networks of ABA-JA synergistic Accepted Article interaction during rice seed germination.