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生物信息学研究

组长:吴建利

电话:0571-63370326

邮箱:beishangd;163.com

所属部门:国家水稻改良中心


利用水稻突变体等资源,开展重要农艺性状的遗传分析、基因克隆和功能验证;并利用标记辅助选择与转基因技术进行分子育种。

研究方向及内容

水稻分子遗传学:利用稻种资源、突变体和遗传群体开展重要农艺性状、主要病害以及类病害的遗传机理研究。通过开展基因定位和克隆分离,解明控制重要农艺性状、病害及类病害基因的功能。

水稻分子育种与生物技术:通过基因精细定位与分离,开发紧密连锁与功能标记,进行有利基因聚合、创制新型育种材料。研究调控相关性状座位的遗传多样性,建立相应基因座位的生物信息数据库,促进重要性状功能基因的育种利用。

科研成果

——发表的主要论文

1. Feng Baohua,Yang Yang,Shi Yongfeng,Shen Haichao,Wang Huimei,Huang Qina,Xu Xia,Lv Xiangguang,Wu Jianli.Characterization and Genetic Analysis of a Novel Rice Spotted-leaf Mutant HM47 with Broad-spectrum Resistance to Xanthomonas oryzae pv. oryzae.Journal of Integrative Plant Biology,2013,55(5):473-483.

2. Reena Narsai,Wang Chuang,Chen Jie,Wu Jianli,Shou Huixia,James Whelan.Antagonistic, overlapping and distinct responses to biotic stress in rice (Oryza sativa) and interactions with abiotic stress.BMC Genomics,2013,14(93):1-21.

3. Feng Baohua,Yang Yang,Shi Yongfeng,Lin Lu,Chen Jie,Wei Yanlin,Hei Leung,Wu Jianli.Genetic Analysis and Gene Mapping of Light Brown Spotted Leaf Mutant in Rice.Rice Science,2013,20:13-18.

4. Wang Huimei,Chen Jie,Shi Yongfeng,Pan Gang,Shen Haichao,Wu Jianli.Development and Validation of CAPS Markers for Marker-Assisted Selection of Rice Blast Resistance Gene Pi25.Acta Agronomica Sinica,2012,38(11):1960-1968.

5. 奉保华,杨杨,施勇烽,林璐,陈洁,黄奇娜,魏彦林,Hei Leung,吴建利.水稻淡褐斑叶突变体lbsl1的遗传分析与基因定位.中国水稻科学,2012,26(3):297-301.

6. Huang Qina,Shi Yongfeng,Yang Yang,Feng Baohua,Wei Yanlin,Chen Jie,Marietta Baraoidan,Hei Leung,Wu Jianli.Characterization and Genetic Analysis of a Light- and Temperature-sensitive Spotted-leaf Mutant in Rice.Journal of Integrative Plant Biology,2011,53(8):671-681.

7. Chen Jie,Shi Yongfeng,Liu Wenzheng,Chai Rongyao,Fu Yaping,Zhuang Jieyun,Wu Jianli.A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae.Journal of Genetics and Genomics,2011,38:209-216.

8. Shen Weiwei,Song Chengli,Chen Jie,Fu Yaping,Wu Jianli,Jiang Shaomei.Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance.Rice Science,2011,18(1):17-22.

9. Shao Gaoneng,Tang Ao,Tang Shaoqing,Luo Ju,Jiao Guiai,Wu Jianli,Hu Peisong.A new deletion mutation of fragrant gene and the development of three molecular markers for fragrance in rice.Plant Breeding,2011,130(2):172-176.

10. 魏彦林,施勇烽,吴建利.水稻核基因控制的叶色变异.核农学报,2011,25(6):1169-1178.

11. Cao Liyong,Wu Jianli,Fan Yeyang,Cheng Shihua,Zhuang Jieyun.QTL analysis for heading date and yield traits using recombinant inbred lines of indica rice grown in different cropping seasons.Plant Breeding,2010,129(6):676-682.

12. Shi YongFeng,Chen Jie,Liu WenQiang,Huang Qina,Shen Bo,Hei Leung,Wu Jianli.Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.).Science in China Series C: Life Sciences,2009,52(9):885-890.

13. 黄奇娜,杨杨,施勇烽,陈洁,吴建利.水稻斑点叶变异研究进展.中国水稻科学,2010,24(2):108-115.

14. 刘文强,樊叶杨,陈洁,施勇烽,吴建利.通过生育期基因型选择避免稻瘟病抗性与结实率的遗传累赘.中国水稻科学,2008,22(4):359-364.

15. 吴建利,马良勇,刘坚.抗病性分子育种.见:钱前主编:水稻基因设计育种.p111-164,科学出版社,2007。

16. 吴建利,施勇烽,陈洁,Casiana VERA CRUZ,庄杰云,Hei Leung,郑康乐.IR64品种内残余变异的来源和遗传模式.中国水稻科学,2007,21(2):131-135.

17. Wu Jianli,Fan Yeyang,D.-B.Li,Zheng kangle,Hei Leung,Zhuang jieyun.Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates.Theoretical and Applied Genetics,2005,111(1):50-56.

18. Wu Jianli,Wu Chanjian,Lei Cailin,Marietta Baraoidan,Alicia Bordeos,Ma. Reina Suzette Madamba,Marilou Ramos-Pamplona,Ramil Mauleon,Arlett Portugal,Victor Jun Ulat,Richard Bruskiewich,Wang Guoliang,Jan Leach,Gurdev Khush,Hei Leung.Chemical-and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics.Plant Molecular Biology,2005,59(1):85-97.

19. Wu Jianli,P.K.Sinha,M.Variar,Zheng kangle,J.E.Leach,B.Courtois,H.Leung.Association between molecular markers and blast resistance in an advanced backcross population of rice.Theoretical and Applied Genetics,2004,108:1024-1032.

20. J. Ramalingam,C. M. Vera Cruz,K. Kukreja,J. M. Chittoor,Wu Jianli,S. W. Lee,M. Baraoidan,M. L. George,M. B. Cohen,S. H. Hulbert,J. E. Leach,Hei Leung.Candidate Defense Genes from Rice, Barley, and Maize and Their Association with Qualitative and Quantitative Resistance in Rice.Mol.Plant-Microbe Int.,2003,16:14-24.

21. Moumeni A,BY Samadi,Wu Jianli,Hei Leung.Genetic diversity and relatedness of selected Iranian rice cultivars and disease resistance donors assayed by simple sequence repeats and candidate defense gene markers.Euphytica,131(3):275-284.

课题组成员

研究与技术人员:吴建利、张晓波、施勇烽、徐 霞、王惠梅

博士研究生:施勇烽、黄奇娜、奉保华、王惠梅

硕士研究生:吕向光、李小红、郭 丹、宋莉欣、吴国林

 

联 系 人:吴建利

联系电话:0571-63370326    传真:0571-63370326    电子邮箱:beishangd;163.com

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内容更新日期:2013-08-20